BIOL I 100-4
In this practical the lipids present in egg yolk were separated through absorption chromatography using a TLC plate. The concept is similar to paper chromatography, a plate containing a thin hydrophilic layer called a matrix is used, and a non polar solvent is allowed to move up the plate through capillary action. Thus more polar lipids will move more slowly than non polar lipids.
The egg yolk solution was compared against six standards; cholesterol, cholesterol palmitate, trioleine, linoleic acid, phosphatidylcholine, and phosphatidylethanolamine.
The result obtained was that egg yolk contains trioleine, cholesterol palmitate and phosphatidylethonolamine, the latter being the most polar of the three elements hardly moving up the TLC because of the presence of several polar groups; phosphate groups, amine groups and several oxygens , and cholesterol palmitate being the most non polar.
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Again maybe too much about the background and not enough about the results. It doesn't have pages :-)
ReplyDeleteAnyhow, its clear that you get the concept of movement based on differential solubility in the mobile phase, which is basically due to different polarities of the molecules.
/Steve